TABLE 2.

Thermal denaturation temperatures (T_m values) of TprC subfamily proteins, E. coli OmpA, and E. coli OmpF calculated from far-UV CD transition and SYPRO Orange® dye

Transition data refer to the molar ellipticity at 218 nm.

Protein	Tma by CD	Tm by SYPRO® Orange
E. coli OmpF	79.3 ± 0.7
E. coli OmpA	52 ± 1.5
TprCFl	71.5 ± 0.2
TprCN	50.3 ± 0.4	50.0 ± 0.5
TprCC	62.3 ± 0.5
TprIFl	67.2 ± 1.2
TprIN	50. 6 ± 0.4	50.0 ± 0.5
TprIC	62.1 ± 0.5
TprF	51.4 ± 0.8	50.2 ± 0.8

^a T_m values were obtained by non-linear regression analysis to the CD thermal curves.