TABLE 2.
Protein | Tma by CD | Tm by SYPRO® Orange |
---|---|---|
E. coli OmpF | 79.3 ± 0.7 | |
E. coli OmpA | 52 ± 1.5 | |
TprCFl | 71.5 ± 0.2 | |
TprCN | 50.3 ± 0.4 | 50.0 ± 0.5 |
TprCC | 62.3 ± 0.5 | |
TprIFl | 67.2 ± 1.2 | |
TprIN | 50. 6 ± 0.4 | 50.0 ± 0.5 |
TprIC | 62.1 ± 0.5 | |
TprF | 51.4 ± 0.8 | 50.2 ± 0.8 |
a Tm values were obtained by non-linear regression analysis to the CD thermal curves.