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. 2015 Mar 17;290(19):12355–12369. doi: 10.1074/jbc.M114.625012

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Orc1 associates with mitotic chromosomes. Tetracycline-inducible GFP-Orc1 U2OS cell line was followed by time-lapse live cell imaging through mitosis. Highlighted by an arrow is a cell followed through mitosis (panels a–x in A and panels a–u in B) at indicated time points (min means minutes, and h means hours). Orc1 is loaded onto chromatin during prophase and stays on chromatin throughout mitosis, after telophase Orc1 becomes diffusely distributed in daughter nuclei (panels v–x). Scale bar denotes 5 μm. A and B represent images from two separate videos.