FIGURE 6.

IL-1β does not influence migration but enhances binding of monocytes to keratinocytes. A, scratch wounding of confluent HaCaT cultures was followed by the addition of fresh medium with or without IL-1β (10 ng/ml). The closure of the wound area was followed by image analysis. Means ± S.E. (error bars) are shown from three independent experiments, each performed with six replicates. The effect of IL-1β was not statistically significant (ANOVA). B and C, HaCaT cultures were treated with IL-1β (10 ng/ml) and KN93 (10 μm) for 20 h and analyzed for hyaluronan-dependent monocyte binding as explained under “Experimental Procedures.” The data represent means ± S.E. from seven (B) and five (C) experiments. D and E, cells were transfected with wild type and the S325D mutant of CD44. D, the efficacy of transfections was confirmed in Western blots with Hermes 3 antibody. E, monocyte binding to the cultures was assayed 48 h after transfections. Cultures transfected with an empty vector were set as 100%. The data represent means ± S.E. from four independent experiments. Statistical significance of the differences was tested using ANOVA and Dunnett's post hoc test or a one-sample Student's t test; *, p < 0.05; **, p < 0.01.