TABLE 1.
Identification of donkey milk fat globule membrane proteins by PMF
| Spot no. (from Fig. 1) | Protein name | UniProt KB/TrEmbl entry | MOWSE scorea | Experimental/nominal molecular mass | PMF number of signals m/z matched/number of signals m/z collected (% sequence coverage) |
|---|---|---|---|---|---|
| Da | |||||
| 1 | Milk fat globule-EGF factor 8 splice variant | F5CEP2 | 5.25e + 8 | 50,000/43,308 | 24/78 (66.2%) |
| 2 | Milk fat globule-EGF factor 8 splice variant | F5CEP2 | 2.26e + 9 | 50,000/43,308 | 23/59 (61.1%) |
| 3 | Milk fat globule-EGF factor 8 splice variant | F5CEP2 | 4.74e + 6 | 45,000/43,308 | 17/60 (54.1%) |
| 4 | Milk fat globule-EGF factor 8 splice variant | F5CEP2 | 1.44e + 7 | 45,000/43,308 | 18/54 (54.1%) |
a MOWSE score is obtained by the MS-Fit search and concerns the highest scored protein.