TABLE 3.
Identification of the 45-kDa donkey lactadherin short form by enzymatic digestions and mass spectrometry techniques
| Enzyme | PMF by MALDI-TOF |
LC-MS/MS |
||||
|---|---|---|---|---|---|---|
| Position | m/z, single charge | Sequencea | Position | m/z, charge state | Sequencea | |
| Trypsin | 127–134 | 838.4305 | GGTAEYLK | 89–102 | 770.3600 (+2) | TGIVNAWTASNYDK |
| 138–144 | 866.4367 | VAYSVDGR | 289–300 | 706.2300 (+2) | DFGHIQYVAAYK | |
| 116–126 | 1074.5902 | VTGVVTQGASR | 25–37 | 801.7400 (+2) | GDVFTQYICSCPR | |
| 349–357 | 1105.6265 | ILPVAWHNR | 278–288 | 558.2200 (+2) | QVTGVVTQGAR | |
| 358–367 | 1173.6660 | ITLRVELLGCam | 160–171 | 624.6400 (+2) | VFVGNVDNSGLK | |
| 336–345 | 1225.6034 | NMFETPFLAR | 116–126 | 537.7100 (+2) | VTGVVTQGASR | |
| 336–345 | 1241.5983 | NMoxFETPFLAR | 199–224 | 959.2800 (+3) | FELLGCEVNAGCAEPLGLEDNSIPDR | |
| 289–300 | 1411.7005 | DFGHIQYVAAYK | 349–357 | 553.1500 (+2) | ILPVAWHNR | |
| 146–157 | 1411.7328 | KFQFIRDAGDSK | 173–185 | 854.8500 (+2) | VNMFDVPLEVQYVR | |
| 323–335 | 1483.7625 | IFPGNLDNNSHKK | 255–277 | 856.5900 (+3) | FNAWTAQSNSASEWLQVDLGSQK | |
| 186–198 | 1519.7620 | LVPVACamHHGCamTLR | 186–198 | 760.2300 (+2) | LVPVACHHGCTLR | |
| 25–37 | 1602.7039 | GDVFTQYICamSCamPR | 336–345 | 613.2900 (+2) | NMFETPFLAR | |
| 172–185 | 1708.8727 | VNMFDVPLEVQYVR | 323–334 | 678.1600 (+2) | IFPGNLDNNSHK | |
| 103–115 | 1717.8989 | NPWIQVNLMRKMR | 79–85 | 435.6300 (+2) | WVPELAR | |
| 172–185 | 1724.8676 | VNMFDVPLEVQYVR | ||||
| 234–248 | 1878.8962 | TWGLNAFSWYPFYAR | ||||
| 255–277 | 2567.2161 | FNAWTAQSNSASEWLQVDLGSQK | ||||
| 199–224 | 2875.3237 | FELLGCamEVNAGCamAEPLGLEDNSIPDR | ||||
| Chymotrypsin | 347–354 | 953.5931 | VRILPVAW | |||
| 233–240 | 964.4999 | RTWGLNAF | ||||
| 32–39 | 1012.4339 | ICamSCamPRGY | ||||
| 87–95 | 1053.5588 | HRTGIVNAW | ||||
| 71–79 | 1157.5561 | FGFMGLQRW | ||||
| 246–255 | 1225.6688 | YARLDKQGKF | ||||
| 76–86 | 1267.7270 | QRWVPELARL | ||||
| 29–39 | 1404.6035 | TQYICamSCamPRGY | ||||
| 246–258 | 1596.8281 | YARLDKQGKFNAW | ||||
| 296–310 | 1618.7608 | VAAYKVSHSNDGANW | ||||
| 184–197 | 1618.8305 | VRLVPVACamHHGCamTL | ||||
| 148–161 | 1625.8282 | QFIRDAGDSKDKVF | ||||
| 329–342 | 1708.7748 | DNNSHKKNMFETPF | ||||
| 215–232 | 1966.9352 | GLEDNSIPDRQITASSTY | ||||
| 162–183 | 2453.2017 | VGNVDNSGLKVNMFDVPLEVQY | ||||
| 40–71 | 3393.4742 | TGTHCamETTCamAMPLGMETGAIADAQISASSVYF | ||||
| 314–342 | 3393.6393 | RDQRAADSKIFPGNLDNNSHKKNMFETPF | ||||
| 101–132 | 3578.8319 | DKNPWIQVNLMRKMRVTGVVTQGASRGGTAEY | ||||
| Asp-N | 155–165 | 1207.6317 | DSKDKVFVGNV | 272–288 | 872.3800 (+2) | DLGSQKQVTGVVTQGAR |
| 166–175 | 1140.5354 | DNSGLKVNMF | 289–305 | 968.8900 (+2) | DFGHIQYVAAYKVSHSN | |
| 180–198 | 814.3400 (+3) | EVQYVRLVPVACHHGCTLRF | ||||
| 131–142 | 724.3000 (+2) | EYLKTFKVAYSV | ||||
| 166–175 | 570.7000 (+2) | DNSGLKVNMoxF | ||||
| 26–44 | 774.8400 (+3) | DVFTQYICSCPRGYTGTHC | ||||
| 158–165 | 439.2100 (+2) | DKVFVGNV | ||||
| 166–175 | 562.6800 (+2) | DNSGLKVNMF | ||||
| 320–328 | 495.7300 (+2) | DSKIFPGNL | ||||
| 45–60 | 842.7800 (+2) | ETTCAMoxPLGMoxETGAIA | ||||
| 143–151 | 583.7300 (+2) | DGRKFQFIR | ||||
| 13–19 | 415.1600 (+2) | DGECHVI | ||||
| 250–266 | 920.3900 (+2) | DKQGKFNAWTAQSNSAS | ||||
| 1–12 | 674.6700 (+2) | ASGPCFPNPCQN | ||||
| 45–60 | 834.8300 (+2) | ETTCAMoxPLGMETGAIA | ||||
a Cam, carbamidomethylated cysteine; Mox, oxidized methionine.