FIGURE 6.

Overexpression of miR-7 or knockdown of RelA increases expression of the glucose transporter Glut3. A and B, SH-SY5Y cells were transfected with miR-SC or miR-7 (A) and siRNA-NT or siRNA-RelA (B). After 48 h, cells were harvested, followed by RNA extraction and qPCR to quantify expression of glycolytic genes Glut1, Glut3, Glut4, Eno1, PGK1, PDK4, and SCO2. C, SH-SY5Y cells were co-transfected as indicated. After 48 h, cells were treated with 2 mm MPP⁺ for 24 h. Cell viability was quantified using the MTS assay. D, qPCR data showing efficiency of Glut3 knockdown after transfection of siRNA-Glut3. For A, B, and C, data were analyzed using two-way analysis of variance followed by Bonferroni's post hoc test. For D, data were analyzed using Student's t test. Data are presented as means ± S.E. ***, p < 0.001. These results are representative of three separate experiments.