Table III. List of proteins identified in Bradyrhizobium japonicum by LCMS/MS from the soybean SM. A minimum of two unique peptides were identified in one or more biological samples for all proteins indicated. Symbiosome membrane (SM) (bicarbonate stripped and chloroform-methanol extractions pooled). Percentage coverage (%C) is the maximum percentage of a protein to which peptides have been mapped in a biological sample. Membrane topology has been predicted by three bioinformatic suites: (i) SOSUI, (ii) TMHMM and (iii) TopPred2, with number of predicted Trans-Membrane Domains indicated.

Accession number	Description	SM	%C	i	ii	iii
gi|27383032	ABC transporter substrate-binding protein	2	16	0	0	1
gi|384213970	Acetyl-CoA acetyltransferase	7	29	0	0	1
gi|27376888	AhpC gene product	5	19	0	0	0
gi|27378020	Amino acid binding protein	6	61	0	1	2
gi|1209038	FixA	3	11	0	0	0
gi|27377170	GroEL gene product	85	12	0	0	0
gi|384221462	GroES gene product	69	12	0	0	0
gi|27376941	Hypothetical protein	23	51	0	0	0
gi|27380266	Hypothetical protein	19	9	0	0	0
gi|27381635	Hypothetical protein	10	8	0	0	3
gi|12620578	ID352	9	10	0	1	1
gi|12620644	ID525	10	30	0	0	4
gi|12620707	ID693	30	5	1	0	2
gi|27376854	NifD gene product	23	12	0	0	0
gi|27376880	NifH gene product	68	9	0	0	0
gi|152324	Nitrogen fixation protein	16	3	0	0	0
gi|27376422	Outer membrane protein	9	12	0	1	2
gi|27376315	Outer-membrane immunogenic protein	7	7	1	2	1
gi|27379812	Outer-membrane immunogenic protein	12	9	2	0	0
gi|27379978	Outer-membrane immunogenic protein	41	11	0	1	0
gi|27382806	Outer-membrane immunogenic protein	59	7	0	1	0
gi|27382260	Peptidoglycan-associated lipoprotein	17	1	0	0	1
gi|27382263	TolB gene product	5	7	0	0	1