Fig. 1.

Global analysis of the results of the screening. A, Scheme of the translocation criteria upon NK2R-HEK cell activation with the neurokinin A agonist (100 nm). Examples of images extracted from the videos displaying fluorescent proteins in the cytoplasm (PKCA-YFP), in the nucleus (CFP-FAM58A), at the plasma membrane (PKCA-YFP upon NKA activation of the cells), in intracellular structures (ARRB2-YFP after NKA activation). B, Classification and statistics of the 193 proteins screened according to 15 categories based on known cellular processes. C, Classification of the 53 proteins displaying a specific localization before activation according to the 15 categories. D, Classification of the eight proteins that respond to NKA activation according to the 15 categories. Scale bar 5 μm.