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. 2015 Apr 29;15:324. doi: 10.1186/s12885-015-1315-9

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© Ito et al.; licensee BioMed Central. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Nuclear and cytoplasm p65 protein expression in T24 and T24PR cells. The extracted nucleus and cytoplasmic protein (20 μg) were immunoblotted with p65 antibody. Lamin A/C was used as a loading control for nuclear extraction, and β-actin was used as a loading control for cytoplasm extraction.