Figure 1.

Overview of the workflow for construction of IR-injury-associated regulatory networks. In the first step (I), we collected IR-related miRNAs, TFs and mRNAs from the experimental mRNA- and miRNA-arrays produced in our laboratory. These represent the altered expression values of the 3 elements detected at 3 different time points during ischemia-reperfusion injury. We then constructed TF-mRNA pairs, miR-mRNA pairs, and miR-TF pairs with the aid of external databases and/or software (II). The paired constructs were used to build novel closed loop-motifs consisting of 3 nodes relationally interconnected by 3 edges (III). The motifs were further integrated into IR-injury associated regulatory networks that consist of interconnected loop-motifs (IV). Green filled-circles denote miRs, red filled-circles denote TFs and blue filled-circles denote target genes (transcripts).