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. 2015 Apr 3;13:29. doi: 10.1186/s12951-015-0088-2

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© Maiolino et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Cytotoxicity of unloaded NPs. A549 and Calu-3 cells were exposed to increasing concentrations of PLGA/PEI/HA and PLGA/PEI NPs for 24 h. After incubation, cell viability and released LDH were evaluated using the MTT (A, B) and LDH (C, D) assays. The cell viability and LDH release from untreated control were set to 100% and 0%, respectively. Results are presented as percentage (mean ± SEM) (n = 3) of the control cells. Differences were considered not statistically significant (P >0.05).