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Cytotoxicity of DTX loaded-NPs in A549 cells. A549 cells were exposed to increasing concentrations of free DTX, DTX-PLGA/PEI/HA NPs or DTX-PLGA/PEI NPs for 72 h. After incubation, cell viability and released LDH were evaluated using the MTT (A, B) and LDH (C, D) assays. The cell viability and LDH release from untreated cells were set to 100% and 0%, respectively. Results are presented as percentage (mean ± SEM) (n = 3) of the control cells. *P <0.05, # P < 0.001.
