Table 1.
Properties of DTX-loaded NPs before and after freeze-drying
DTX-PLGA/PEI NPs | DTX-PLGA/PEI/HA NPs | DTX-PLGA/PEI NPs freeze-dried | DTX-PLGA/PEI/HA NPs freeze-dried | |
---|---|---|---|---|
Trehalose/NP weight ratio | - | 60 | ||
Yield (% ± SD) | 21.2 ± 0.8a | - | ||
Mean DH (nm ± SD) | 155 ± 9b | 173 ± 10b | 176 ± 4c | 203 ± 10c |
Polydispersity index | 0.10b | 0.13b | 0.20c | 0.17c |
Zeta Potential (mV) | +39.0b | −23.2b | +33.5c | −22.3c |
DTX actual loading(mg per 100 mg) | 0.95 ± 0.5 | 0.013 ± 0.001 | ||
PEI actual loading(mg per 100 mg) | 7.94 ± 2.1 | 0.132 ± 0.010 | ||
HA actual loading(mg per 100 mg) | - | 12.2 ± 0.8 | - | 0.20 ± 0.03 |
aCalculated as ratio between total component weight and NP weight.
bAs prepared NPs before freeze-drying.
cAfter dispersion of freeze-dried powder in water.