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. 2015 Apr 14;12:62. doi: 10.1186/s12985-015-0293-5

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© Rajput et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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c-Myc- dependent transcriptional regulation of UBF in the presence of HBx. (A) Huh7 cells were transiently transfected with vector, HBx and/or Myc- shRNA plasmids. Total RNA was isolated and the levels of UBF mRNA were measured by RT-qPCR. (B) IHH cells were transiently transfected with vector or HBx expression plasmids. After 48 h, cells were subjected to ChIP assay using 'anti-Myc antibody. Pre-immune sera were used as a negative control. Fold DNA enrichment over mock due to UBF occupancy was measured by ChIP-qPCR using primers specific for UBF promoter (Additional file 1: Table S1). Data are shown as mean ± S.D of three independent experiments. * represents a statistically significant difference of p <0.05.