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. 2015 Mar 22;12:46. doi: 10.1186/s12985-015-0279-3

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© Rawle et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Replication of WNV _NY99 and WNV _NSW2011 in human MoDCs. MoDCs from donors 1 (a, b), 2 (c, d) and 3 (e, f) were infected with WNV_NY99 and WNV_NSW2011 at MOI = 1. Cell culture supernatant was collected at 24, 48 and 72 hours post-infection and virus titer was determined by plaque assay on BHK cells (a, c, e). Total cellular RNA was collected at 24, 48 and 72 hpi and vRNA levels as determined by qRT-PCR were normalised to a combination of three endogenous controls (TBP, GAPDH and PPIA), and was expressed as fold change from uninfected samples (b, d, f). The data from all donors (donors 1 to 3) were combined and error bars represent standard error of the mean (g, h). A Student’s t-test was performed to determine statistical significance at each time point (*: P ≤ 0.05).