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. 2015 Mar 22;12:46. doi: 10.1186/s12985-015-0279-3

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© Rawle et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Early IFN/ISG response to infection with WNV _NY99 and WNV _NSW2011 in human MoDCs. MoDCs from three separate donors were infected with WNV_NY99 or WNV_NSW2011 at MOI = 1 and total cellular RNA was isolated at 24 hours post-infection. vRNA (a), IFNβ (b), OAS1 (c) and MxA (d) mRNA levels as determined by qRT-PCR were normalised to a combination of three endogenous controls (TBP, GAPDH and PPIA), and expressed as fold change from uninfected samples. The results show mean values from three different donors (donors 1 to 3) and error bars represent standard error of the mean. A Student’s t-test was performed to determine statistical significance (*: P ≤ 0.05).