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. 2015 May 8;5:9903. doi: 10.1038/srep09903

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(a) BMP7 and EREG had no direct effect on the gene expression of oral epithelial cells. (b) qPCR detected the relative gene-expression levels under 3-D co-culturing conditions with different supplementation. OEs co-cultured with ADPCs without supplementation showed no AMLX expression. OEs co-cultured with ADPCs supplemented with BMP7 and EREG showed positive and significantly increased AMLX, AMBN, and AMTN expression. KRT14 expression accordingly decreased after supplementation. (c) Immunofluorescence staining in OEs showed positive signals for amelogenin after BMP7 and EREG supplementation. Scale bar: 20 μm. (d) Odontogenic-associated transcriptional factors and mature markers of ameloblasts were tested. PITX2 expression was significantly higher in OEs co-cultured with FDPCs and ADPCs supplemented with BMP7/EREG compared with OEs co-cultured with ADPCs. LEF1 expression significantly decreased in OEs co-cultured with ADPCs.