(Top) Temporal evolution of the segregation parameter Φ. DUPC/x/chol membranes where x = DLPC (black), DPPC (red), DSPC (green). Solid lines correspond to chlf-free systems, whereas dashed lines stand for bilayers with 0.77 chlf per lipid. (Bottom) Sequence of the phase segregation process observed in one of the leaflets of the simulated DUPC/DPPC/chol membrane system in the absence of chlf. Voronoi polygons are filled with a colour that identifies each one of the lipid components: DUPC (blue), DPPC (red) and chol (orange). DPPC and chol segregates in a more packed phase (lo) than the one formed by DUPC (ld). From left to right, t = 0, 0.4, 1.6 and 12 µs.