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. 2015 Apr;10(4):651–658. doi: 10.4103/1673-5374.155442

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Copyright: © Neural Regeneration Research

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effect of hUCMSC-conditioned medium on neurite outgrowth from DRG explants.

(A) Morphology of DRG explants and staining for NF200 after 72-hour treatment with control medium, hUCMSC-conditioned medium or 50 ng/mL NGF. Scale bars: 500 μm. The neurite outgrowth distance (B) and percentage of axon area/DRG area (C) of the DRG explants were measured and calculated under a fluorescence microscope. All data are expressed as the mean ± SD. Statistical analysis was performed using one-way analysis of variance followed by Tukey's test. **P < 0.01, vs. blank medium. hUCMSCs: Human umbilical cord-derived mesenchymal stem cells; DRG: dorsal root ganglion; NF200: neurofilament 200; NGF: nerve growth factor.