Figure 4.

Characterization of F4/80⁺ cells in the tubulointerstitium of kidneys from complement factor H (CfH)^−/− mice chimeric for wild-type (WT), CfH^−/−, FcγR^−/−, and CD11b^−/− bone marrow immunized for 5 weeks with apoferritin, or saline as control. (a) Representative data for Ly6C (y-axis) and CCR2 (x-axis). The four quadrants are boxed and numbered; the percentage each group comprises of total F4/80⁺ cells from the representative graphs is provided in tabular form in b (in the columns ‘Example'). Data from all animal kidneys are also provided as ‘Mean±s.e.m.' in the adjacent columns. *P<0.01 versus all other groups (one-way analysis of variance followed by Tukey's all-pairwise testing). (c) Ly6C^hiCCR2^hi M1 macrophages (quadrant 2, red arrows) were analyzed for CD11c (y-axis) and CD11b (x-axis) staining. The red arrowhead depicts Ly6C^hiCCR2^hiCD11c⁺CD11b⁻ cells in CD11b^−/− chimeras.