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. 2015 May 8;9:176. doi: 10.3389/fncel.2015.00176

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Copyright © 2015 Natsubori, Takata and Tanaka.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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KENGE-tet system requires tetO cassete knockin. Actb gene structure and insertion site of tetO cassette. Mouse Actb gene consists of 6 exons (rectangles), and the tetO cassette (yellow) is inserted downstream of the polyadenylation signal of Actb gene. TetO is the cassette containing the tetracycline operon site (closed squares) and CMV minimal promoter (circle P). AATAAA is a polyadenylation signal. Knocking-in of tetO cassette into the euchromatic locus is a key point for the achievement of higher expression with tTA. Actb: β-actin, cDNA: complementary DNA, pA: ployadenilation signal, tetO: tet operator, tTA: tetracycline transactivator.