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. 2015 May 8;6:422. doi: 10.3389/fmicb.2015.00422

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Copyright © 2015 Metselaar, den Besten, Boekhorst, van Hijum, Zwietering and Abee.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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rpsU and upstream region sequence alignment of Sanger sequenced L. monocytogenes LO28 and acid resistant variants. The upper alignment represent the nucleotide sequence of the region where mutations were found. The black line indicates the start codon of the rpsU gene. The five sequences below the WT are the variations of rpsU found in several variants (indicated with the numbers). The lower alignment represents the amino acid sequence of the complete rpsU gene and the effect of the mutations on the amino acid sequence.