Figure 3.

Analysis of purified nucleotide sugars by HPLC. (A) Nucleotide sugars were extracted and the purified material from CHO-K1 (wild-type) or CHO-G9C (CAD defective) cells with or without 30 µm uridine treatment were run on HPLC. (B) Nucleotide sugars were extracted in the same manner as above and the purified material from GM00038, GM03348 and UDP4003 with or without 30 µm uridine treatment was run on HPLC. The control sample represents an average of both GM00038 and GM03348. In both (A) and (B), the bar graph represents the peak area for each metabolite normalized to ATP levels, since ATP were consistently equal in multiple experiments. Error bars show standard deviation.