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. 2015 Feb 12;24(11):3050–3057. doi: 10.1093/hmg/ddv057

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Figure 5. — Expression and complementation assays. (A) Western blot analysis of HEK 293T cells transfected with FLAG tagged CAD expression constructs showing reduced expression of the exon-13 deleted CAD and normal expression of the pR2024Q CAD, when compared with wild-type CAD. (B) CHO-G9C cells transfected with FLAG tagged CAD constructs showing their ability to rescue the growth defect in the absence of uridine. In the non-rescued samples there is minimal growth, with any detectable growth is likely due to low levels of uridine with in the serum.