Figure 4.
Functional analysis of EDC3 variant. EDC3-DCP2 decapping assay. Wild-type EDC3 enhances DCP2-mediated decapping in vitro, while a similar enhancement was not observed with the Phe54Ser variant (EDC3F54S), and inhibition of decapping was detected at the highest concentration tested. Decapping assays were carried out with 32P-cap-labeled RNA with 10 nm DCP2 and the indicated amounts of EDC3 proteins as previously described (24). (A) A representative decapping assay is shown and labeled as in the legend to Figure 3A. A schematic of the substrate with the m7Gppp denoting the cap and the line representing the RNA is shown at the bottom. (B) The average of three independent decapping assays in the presence of the different EDC3 proteins is shown with error bars representing ±standard deviation.