Table 1.

The Primers Used for the RT-PCR Analysis

Target gene	Primers	Sequence	Fragment size (bp)
β-Actin	Forward	5′-GGGCTGTATTCCCCTCCATCG-3′	201
	Reverse	5′-GCAGCTCATTGTAGAAGGTGTGGTG-3′
Rev-erbα	Forward	5′-ATTCGGGAGGTGGTAGAGTTTGC-3′	148
	Reverse	5′-ATCACTGTCTGGTCCTTCACGTTG-3′
Bmal1	Forward	5′-AACCTTCCCGCAGCTAACAG-3′	79
	Reverse	5′-AGTCCTCTTTGGGCCACCTT-3′
Wnt3a	Forward	5′-CTTAGTGCTCTGCAGCCTGA-3′	92
	Reverse	5′-GAGTGCTCAGAGAGGAGTACTGG-3′
β-Catenin	Forward	5′-GGGTCCTCTGTGAACTTGCTC-3′	165
	Reverse	5′-TGTAATCCTGTGGCTTGTCCTC-3′
GSK-3β	Forward	5′-CCTTATCCCTCCACATGCTCG-3′	103
	Reverse	5′-GTTATTGGTCTGTCCACGTCTC-3′
OSX	Forward	5′-TGCCTACTTACCCATCTGACTT-3′	134
	Reverse	5′-TTGCCCACTATTGCCAACC-3′
ALP	Forward	5′-CCCCCCGTGGCAACTCTATCTT-3′	272
	Reverse	5′-GTAGTTCTGCTCGTGGACGCCG-3′
BSP	Forward	5′-CACAAGCAGACACTTTCACTCC-3′	77
	Reverse	5′-TCCATAAGCCAAGCTATCACC-3′
c-Myc	Forward	5′-CTGTATGTGGAGCGGTTTCTC-3′	90
	Reverse	5′-AGGCTGGTGCTGTCTTTGC-3′
TCF1	Forward	5′-CTACAGCGACGAGCACTTTTCTC-3′	115
	Reverse	5′-GTAGAAGGTGGGGATTTCAGGAG-3′

ALP, alkaline phosphatase; BSP, bone sialoprotein; GSK, glycogen synthase kinase; OSX, Osterix; RT-PCR, reverse transcription–polymerase chain reaction; TCF, T cell factor.