Figure 6.

Melanogenesis and 1,25(OH)₂D₃ modulate VDR, CYP24A1 and CYP27A1 gene expression in B16-F10 melanoma cells. 1,25(OH)₂D₃ was used at a concentration of 1 μM. PCR analysis was performed as described in Materials and Methods, β-actin (ACTB) was used as a control (A); The lengths of amplicons fragments (in base pairs) were: Actin (353), VDR (234), CYP24A1 (201), CYP27A1 (209). Densitometric analysis of PCR results obtained using TotalLab Quant v 11.4 (B,C). NP, non-pigmented cells (F10); NPV, non-pigmented cells treated with 1,25(OH)₂D₃; P, pigmented cells (DMEM); PV, pigmented cells treated with 1,25(OH)₂D₃. Data are from the most representative measurement.