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. 2015 Mar 24;16(4):6677–6693. doi: 10.3390/ijms16046677

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Elevated expression levels of MALAT-1 in pancreatic cancer cells induced endothelial cell migration and tube formation. (A) Transwell migration assay showed that conditioned media from M-si groups decreased HUVEC migration significantly compared to those from M-nc groups (Scale bar, 25 μm); (B,C) HUVEC tube formation assay. Significant decreases in the tube number (B) and tube length (C) were observed in conditioned media from M-si groups compared with those from M-nc groups (Scale bar, 200 μm); (D) VEGF concentration was detected by ELISA. ELISA values were corrected for total cell protein; (E) Western blotting analysis was employed to detect VEGF protein expression of cells lysate. Data are shown as mean ± SD. * p < 0.05 compared with the control group.