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(A) TIRFM images of single Atto-Oxa12 molecules detected in the stratum corneum and in H2O: (A) individual raw image, showing an Atto-Oxa12 molecule in human SC; (B) Gaussian filtered image of (A); (C) Two single Atto-Oxa12 molecules in H2O are shown for comparison; (D) Gaussian filtered image of (C).
