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. 2015 Mar 27;16(4):7027–7044. doi: 10.3390/ijms16047027

Figure 5.

Figure 5

Caspase involvement in cell death. (A) B16F1 cells were pre-incubated with 40 μM ZVAD-FMK 40 min before treatment with 50 μg/mL P1G10 for 24 h. Propidium iodide was added and nuclear fluorescence scored in a FACS flow cytometer. DNA fragmentation was analyzed using FlowJo Software. Data are shown as mean ± SD and (B) Representative histograms of the sub-diploid content after P1G10 treatments in the presence or absence of ZVAD-FMK are shown (*** p < 0.001, ANOVA, Bonferroni’s post-test).