Table 1.

Extraneous P. aeruginosa BK-76 cell swimming metrics. Center of mass describes the overall change in position of the cell group’s center. Increasingly negative X values indicate that cell group has more efficiently migrated along the VEGF concentration gradient (towards highest concentration). Negative values for the center of mass X are due to the microfluidic device orientation, as the VEGF concentration gradients were generated from left (negative) to right (positive) across the chemotaxis area. Thus, a cell group’s shift towards the VEGF source would be shown as a negative value. Accumulated and Euclidean distances are used in determining the directness of cell motility. Accumulated distance represents the total distance travelled. Euclidean distance represents the distance between the start and end point of a cell’s trajectory.

Characteristics	Baseline	0.1 ng/mL	1 ng/mL	10 ng/mL
Center of Mass X	−0.98	−0.69	−2.39	−5.87
Center of Mass Y	0.52	−0.08	−0.81	−0.75
Accumulated Distance (μm)	26.30 ± 8.63	23.78 ± 12.72	19.02 ± 9.35	17.83 ± 9.49
Euclidean Distance (μm)	5.68 ± 3.03	6.89 ± 2.86	6.34 ± 3.15	7.61 ± 3.76