Table 2.
DNA barcoding performance evaluated based on similarity methods per ITS dataset per subgenus.
| Subgenus | Barcode Region | N Individuals | BM (%) | BCM (%) | Threshold (%) | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| Correct | Ambiguous | Incorrect | Correct | Ambiguous | Incorrect | No Match | ||||
| Astrophea | ITS1 | 53 | 73.58 | 5.66 | 20.75 | 73.58 | 3.77 | 5.66 | 16.98 | 1.51 |
| ITS2 | 53 | 73.58 | 3.77 | 22.64 | 73.58 | 3.77 | 9.43 | 13.20 | 2.97 | |
| ITS1+2 | 53 | 73.58 | 3.77 | 22.64 | 73.58 | 3.77 | 5.66 | 16.98 | 1.92 | |
| Decaloba | ITS1 | 314 | 63.05 | 4.13 | 32.80 | 61.78 | 3.82 | 23.24 | 11.14 | 3.77 |
| ITS2 | 314 | 50.95 | 16.87 | 32.16 | 50.31 | 16.55 | 25.15 | 7.96 | 3.46 | |
| ITS1+2 | 314 | 64.64 | 1.27 | 34.07 | 64.01 | 1.27 | 23.88 | 10.82 | 3.43 | |
| Deidamioides | ITS1 | 101 | 96.03 | 0 | 4.96 | 95.04 | 0 | 0 | 4.95 | 2.98 |
| ITS2 | 101 | 96.03 | 0 | 4.96 | 95.04 | 0 | 0 | 4.95 | 5.41 | |
| ITS1+2 | 101 | 96.03 | 0 | 4.96 | 95.04 | 0 | 0 | 4.95 | 3.56 | |
| Passiflora | ITS1 | 287 | 81.53 | 1.74 | 16.72 | 78.39 | 1.39 | 5.57 | 14.63 | 1.83 |
| ITS2 | 287 | 54.70 | 28.57 | 16.72 | 50.87 | 27.87 | 9.40 | 11.84 | 1.19 | |
| ITS1+2 | 287 | 81.18 | 1.74 | 17.07 | 77.00 | 1.39 | 4.52 | 17.07 | 1.28 | |
BM, best match, and BCM, best close match (according to [53]) obtained in TaxonDNA software.