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. 2015 Apr 2;16(4):7377–7393. doi: 10.3390/ijms16047377

Figure 1.

Figure 1

Figure 1

Expression of glucokinase (GCK) and glucokinase regulatory protein (GCKR) in co-transfected liver L-02 cells. GCK and GCKR expressing plasmids were co-transfected into L-02 cells at a range of plasmid ratios (molar) from 1:1 to 1:8. Cells transfected with the GCK expressing, GCKR expressing, and GCKR backbone plasmids were used as controls. (A) Quantitative real-time RT-PCR assessment of the mRNA levels of Gck (open bars) and Gckr (solid bars) at different plasmid ratios; (B) GCK enzymatic activity at different plasmid ratios; (C) Representative Western blots for GCK and GCKR, with β-actin used as a loading control, at different plasmid ratios; and (D,E) Quantification of the immunoblots for GCK (D) and GCKR (E), with relative units for GCK (D) or GCKR (E) abundance being the abundance of GCK or GCKR normalized to the β-actin level for that sample. Data are presented as means ± S.D. (Standard Deviation) (n = 4).