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. 2015 Apr 2;16(4):7377–7393. doi: 10.3390/ijms16047377

Figure 3.

Figure 3

Interaction of GCK and GCKR in whole cell lysates, and nuclear fractions, of co-transfected L-02 cells. L-02 cells were transfected with GCKR and/or GCK expressing plasmids. Total protein cellular lysates were immunoprecipitated with anti-GCKR (A,C,E,F) or anti-GCK (B,D) followed by immunoblotting with anti-GCK and anti-GCKR. Cytoplasmic and nuclear protein cellular lysates were immunoprecipitated with anti-GCKR followed by immunoblotting with anti-GCK and anti-GCKR (A,G). Immunoprecipitation of GCKR in the co-transfected L-02 cells; (B) Immunoprecipitaion of GCK in the co-transfected L-02 cells; (C) Immunoprecipitation of GCKR in GCKR plasmid transfected cells; (D) Immunoprecipitation of GCK in GCK plasmid transfected cells; (E,F) No immunoprecipitation of GCK and GCKR in cells transfected by the backbone vector (E) and in untransfected L-02 cells (F,G). Cytoplasmic and nuclear immunoprecipitation of GCKR in the co-transfected L-02 cells. Hsp90 and histone deacetylase (HDAC) were used as cytoplasmic and nuclear fraction markers, respectively. Input represents cell lysates before co-IP. Data are representative of four experiments with similar results.