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Alkaline phosphatase (ALP) activity in MC3T3-E1 cells seeded on fibronectin-loaded collagen matrix, at 10 days. Collagen matrices were placed in 24-well plates, and MC3T3-E1 cells were cultured on collagen matrices loaded with integrin-binding fibronectin (iFN) or osteoinductive fibronectin (oFN, 5 μg·mL−1). Inset: image of a collagen matrix. The results are reported as mean ± standard deviation (n = 3). * p < 0.05 compared with iFN-loaded groups or control.
