Figure 5.

Osteogenic differentiation activity of osteoinductive fibronectin (oFN) matrix protein as determined by real-time PCR. MC3T3-E1 cells were cultured on collagen matrices loaded with integrin-binding fibronectin (iFN) or oFN (5 μg·mL⁻¹) in 24-well plates. The mRNA levels of OPN, RUNX2, and Col I were measured, and the relative mRNA level of each gene was normalized to that of GAPDH. The results are reported as mean ± standard deviation (n = 3). * p < 0.05 compared with iFN-loaded groups or control.