Figure 4.

The cell disruption degree and lipid extraction yield in different processes. One-liter algal cell suspension at 20 g/L of biomass concentration was firstly treated by disruption and then the lipid was extracted. The disruption conditions were as follows: “CON”, control, the cells were not disrupted; “US”, ultrasonicated at 600 W for 30 min; “HPH”, homogenized one pass at 60 MPa; “E”, treated by 1% (w/w) papain for 2 h followed by 1% (w/w) cellulase for 3 h; “E + HPH”: Treated by 1% (w/w) papain for 2 h followed by 1% (w/w) cellulase for 3 h and then homogenized one pass at 60 MPa; “E + US”: 1% (w/w) papain for 2 h followed by 1% (w/w) cellulase for 3 h, and then ultrasonically treated at 600 W for 30 min. (a) The disintegration degree of different disruption processes; (b) lipid yields of different disruption processes and (c) the relationship of lipid yield with integration degree.