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. 2015 Apr 14;16(4):8337–8350. doi: 10.3390/ijms16048337

Figure 4.

Figure 4

TRAP staining for measurement of the formation of OCs. (A) Typical OCs, defined as TRAP-positive and multinucleate cells, were observed by microscopy (original magnification 200×); and (B) Five high-power fields were randomly selected for each group and the number of OCs were counted. We then calculated the average number of each group to compare the statistical differences. *** p < 0.001. Each experiment was carried out in triplicates.