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Bone resorption by OCs induced by RANKL and M-CSF. The bone slices were rinsed and left overnight in 1 M ammonium hydroxide to remove all cells. The slices were then washed with PBS and stained with 0.5% toluidine blue. The resorption pits were identified by microscopy (original magnification 200×). Each experiment was carried out in triplicates.
