Skip to main content
. 2015 Apr 14;16(4):8364–8381. doi: 10.3390/ijms16048364

Figure 6.

Figure 6

Effect of R848 (resiquimod)-stimulation on NF-κB activity based on firefly luciferase (FLU). HEK293T cells were transfected with an expression vector (pCMV-PiTLR7 or pCMV-empty) and a reporter vector (pGL4.32(luc2P/NF-κB-RE/Hygro)). Twenty-four hours post-transfection, 2.5 μg/mL of R848 was added to the transfected cells and the induction levels of NF-κB luciferase activity were measured after stimulation for 5 h. Columns represent the mean ± SD of three independent experiments. Asterisks indicate significant differences by a t-test between pigeon TLR7- and empty vector-transfected cells (p < 0.001).