miR-124a expression negatively correlates to Atgl (mRNA) levels in various murine tissues. (A,B) Relative Atgl (mRNA) (A) and protein (B) expression measured in various murine tissues. Atgl (mRNA) level in adipose tissue arbitrarily set to 10; (C) Relative expression of mature miR-124a measured by qRT-PCR. Mature miR-124a level in brain arbitrarily set to 10; (D) Correlation between Atgl (mRNA) and miR-124a expression in various murine tissues; (E) Relative Atgl, Cgi-58 and miR-124a expression measured in murine perirenal as well as gonadal WAT; (F) Relative Atgl, Cgi-58 and miR-124a expression measured in murine perirenal WAT from ad libitum fed or, 12 h overnight fasted, mice; (G) Relative Atgl, Cgi-58 and miR-124a expression measured in murine liver from ad libitum fed or, 12 h overnight fasted or, 12 h overnight fasted and 2 h re-fed mice. Data are shown as average ± standard deviation and represent three independent experiments (A–D) or five independent experiments (E–G). ***
p < 0.001, **
p < 0.01, *
p < 0.05.