IL-1ra attenuated neonatal LPS-induced reduction on the number of TH+ cells in the SN of P70 rat brain. Representative photomicrographs of TH+ (red color) (a,b,c); NeuN+ (green color) cells, or double-labeled cells (yellow) (d,e,f) in the SN areas of the midbrain sections; (a,d) saline-treated group; (b,e) LPS-treated group; (c,f) LPS+IL-1ra group; Scale bar: 40 μm. Stereological quantification of TH+ cells (g); and NeuN+ cells (h) in the SN area were shown in g and h, respectively. The results are expressed as the mean ± SEM of six animals in each group, and analyzed by two-way ANOVA. *
p < 0.05 represents a significant difference for the LPS group as compared with the saline group. # p < 0.05 represents significant difference for the LPS+IL-1ra group as compared with the LPS group.