Table 1.
Survival of microencapsulated Bifidobacterium longum with Eleutherine americana in fresh milk tofu during sequential incubation in simulated human gastric and intestinal juices and refrigeration storage.
| Microencapsulation | Time | Plate Count (log10 CFU g−1) | |||
|---|---|---|---|---|---|
| (Day) | Pre-Gastric Juice | Post-Gastric Juice | Post-Intestinal Juice | Refrigeration Storage | |
| Co-encapsulated cells: alginate-E. americana extract | 0 | 9.46 ± 0.07 aA | 9.40 ± 0.10 aA | 9.54 ± 0.19 aA | 9.44 ± 0.13 a |
| Co-encapsulated cells: alginate-oligosaccharides extract | 9.38 ± 0.09 aA | 9.32 ± 0.11 aA | 9.46 ± 0.04 aA | 9.54 ± 0.10 a | |
| Co-encapsulated cells: alginate-commercial fructo-oligosaccharides | 9.30 ± 0.10 aA | 9.51 ± 0.05 aA | 9.57 ± 0.14 aA | 9.46 ± 0.06 a | |
| Non-encapsulated cells: free cells | 9.42 ± 0.07 aA | 9.42 ± 0.03 aA | 9.34 ± 0.12 aA | 9.45 ± 0.14 a | |
| Co-encapsulated cells: alginate-E. americana extract | 2 | 9.36 ± 0.12 aA | 9.45 ± 0.11 aA | 9.42 ± 0.06 aA | 9.35 ± 0.04 a |
| Co-encapsulated cells: alginate-oligosaccharides extract | 9.24 ± 0.06 aA | 9.32 ± 0.09 aA | 9.36 ± 0.03 aA | 9.41 ± 0.06 a | |
| Co-encapsulated cells: alginate-commercial fructo-oligosaccharides | 9.16 ± 0.12 aA | 9.14 ± 0.04 aA | 9.15 ± 0.19 aA | 9.33 ± 0.14 a | |
| Non-encapsulated cells: free cells | 8.47 ± 0.06 bA | 7.45 ± 0.15 cB | 7.30 ± 0.21 cB | 8.30 ± 0.10 b | |
| Co-encapsulated cells: alginate-E. americana extract | 4 | 9.25 ± 0.05 aA | 8.37 ± 0.11 bB | 8.28 ± 0.09 bB | 9.34 ± 0.07 a |
| Co-encapsulated cells: alginate-oligosaccharides extract | 9.17 ± 0.12 aA | 8.15 ± 0.20 bB | 8.15 ± 0.10 bB | 9.25 ± 0.11 a | |
| Co-encapsulated cells: alginate-commercial fructo-oligosaccharides | 9.25 ± 0.16 aA | 8.21 ± 0.14 bB | 8.21 ± 0.11 bB | 9.29 ± 0.11 a | |
| Non-encapsulated cells: free cells | 8.35 ± 0.04 bA | 6.43 ± 0.00 dB | 6.46 ± 0.07 dB | 8.12 ± 0.15 b | |
| Co-encapsulated cells: alginate-E. americana extract | 6 | 8.38 ± 0.05 bA | 7.36 ± 0.20 cB | 7.24 ± 0.16 cB | 8.36 ± 0.06 b |
| Co-encapsulated cells: alginate-oligosaccharides extract | 9.24 ± 0.10 aA | 8.52 ± 0.13 bB | 8.23 ± 0.07 bB | 9.24 ± 0.12 a | |
| Co-encapsulated cells: alginate-commercial fructo-oligosaccharides | 8.45 ± 0.11 bA | 7.19 ± 0.15 cB | 7.26 ± 0.12 cB | 8.25 ± 0.11 b | |
| Non-encapsulated cells: free cells | 6.42 ± 0.08 cA | 5.34 ± 0.06 eB | 3.56 ± 0.16 eC | 6.24 ± 0.06 c | |
Values are means ± standard deviation from duplicate determinations; Different superscript uppercase letters (A,B) in the same row are significantly different among gastrointestinal conditions (p < 0.05); Different superscript lowercase letters (a–e) in the same column are significantly different among co-encapsulating agents (p < 0.05).