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. Author manuscript; available in PMC: 2015 May 8.
Published in final edited form as: J Steroid Biochem Mol Biol. 2009 Jul 21;117(0):56–66. doi: 10.1016/j.jsbmb.2009.07.003

Table 1.

NQO1 polymorphisms and COMT inhibition imbalance estrogen metabolism in human mammary epithelial cells.

Cells treated with 10 µM E2-3,4-Q Negative controls Positive control Polymorphisms




Analytes (pmol/106 cells) Ro41-0960
(µM)
MCF-10F host MCF-10F
empty vector
NQO1 135Stop NQO1WT NQO1 139 Trp NQO1 187 Ser


Clone 4 Clone 5 Clone 2 Clone 3 Clone 4 Clone 6 Clone 2 Clone 3
Catechol and
  conjugates 4-OHE1/2,
  4-OCH3E1/2
  (group total)
1.53±0.31,
28.95±1.56
(30.51)
1.55±0.17,
28.60±0.61
(30.05)
1.40±0.10,
27.71±0.74
(29.11)
1.21±0.08,
28.35±0.73
(29.56)
1.71±0.12,
68.92±1.95
(70.63)
2.44±0.35,
71.71±2.33
(74.15)
0.93±0.24,
25.66±0.97
(26.59)
1.02±0.23,
24.91±0.67
(25.93)
0.99±0.10,
19.46±3.45
(20.45)
0.95±0.01,
21.04±3.59
(21.99)
3 2.49±0.11,
0.62±0.26
(3.11)
2.21±0.15,
0.71±0.16
(2.92)
2.44±0.05,
0.17±0.04
(2.61)
2.43±0.07,
0.18±0.04
(2.61)
2.19±0.03,
0.66±0.09
(2.85)
3.40±0.20,
0.41±0.03
(3.81)
1.56±0.38
0.12±0.04
(1.68)
1.51±0.37,
0.20±0.03
(1.71)
1.82±0.44,
0.33±0.09
(2.15)
1.64±0.40,
0.23±0.05
(1.87)
Quinone conjugates
  (4-OHE1/2-2-SG,
  4-OHE1/2-2-NAcCys,
  4-OHE1/2-2-Cys)
  (group total)
0.32±0.02,
0.46±0.03,
0.74±0.05
(1.52)
0.28±0.03,
0.45±0.02,
0.89±0.04
(1.62)
0.21±0.04,
0.45±0.02,
0.77±0.03
(1.43)
0.14±0.07,
0.47±0.03,
0.52±0.12
(1.13)
0.19±0.01,
0.29±0.03,
0.70±0.02
(1.18)
0.23±0.02,
0.35±0.08,
0.77±0.03
(1.35)
0.27±0.01,
0.39±0.02,
0.71±0.02
(1.37)
0.30±0.05,
0.51±0.03,
0.79±0.03
(1.60)
0.32±0.02,
0.76±0.03,
0.71±0.02
(1.79)
0.24±0.03,
0.59±0.05,
0.71±0.03
(1.54)
3 0.48±0.1,
0.62±0.05,
1.43±0.09
(2.53)
0.48±0.02,
0.61±0.01,
1.37±0.07
(2.46)
0.34±0.02,
0.63±0.02,
1.31±0.04
(2.28)
0.27±0.07,
0.67±0.06,
0.61±0.13
(1.55)
0.26±0.10
0.81±0.07,
1.00±0.07
(2.07)
0.32±0.02,
0.80±0.08,
1.01±0.10
(2.13)
0.30±0.02,
0.75±0.02,
1.14±0.13
(2.19)
0.35±0.02,
0.81±0.02,
1.10±0.06
(2.26)
0.41±0.03,
0.98±0.01,
1.04±0.10
(2.43)
0.37±0.04,
1.04±0.09,
0.94±0.09
(2.35)
DNA adducts
  4-OHE1/2-1-N7Gua,
  4-OHE1/2-1-N3Ade
  (group total)
0.15±0.01,
0.13±0.01
(0.28)
0.13±0.02,
0.14±0.03
(0.27)
0.16±0.01,
0.14±0.03
(0.30)
0.13±0.01,
0.10±0.03
(0.23)
0.09±0.01,
0.08±0.02
(0.17)
0.10±0.01,
0.09±0.01
(0.19)
0.21±0.01,
0.21±0.04
(0.42)
0.25±0.07,
0.24±0.07
(0.49)
0.27±0.09,
0.25±0.08
(0.52)
0.24±0.07,
0.24±0.07
(0.48)
3 0.54±0.02,
0.58±0.02
(1.12)
0.50±0.06
0.54±0.03
(1.04)
0.52±0.09,
0.50±0.04
(1.02)
0.49±0.05,
0.47±0.06
(0.96)
0.37±0.10,
0.33±0.07
(0.70)
0.36±0.07,
0.36±0.06
(0.72)
0.65±0.07,
0.66±0.07
(1.31)
0.60±0.05,
0.62±0.02
(1.22)
0.89±0.19,
0.85±0.15
(1.74)
0.73±0.12,
0.76±0.06
(1.49)
HHS Vulnerability Disclosure