Table 1.
NQO1 polymorphisms and COMT inhibition imbalance estrogen metabolism in human mammary epithelial cells.
Cells treated with 10 µM E2-3,4-Q | Negative controls | Positive control | Polymorphisms | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Analytes (pmol/106 cells) | Ro41-0960 (µM) |
MCF-10F host | MCF-10F empty vector |
NQO1 135Stop | NQO1WT | NQO1 139 Trp | NQO1 187 Ser | ||||
Clone 4 | Clone 5 | Clone 2 | Clone 3 | Clone 4 | Clone 6 | Clone 2 | Clone 3 | ||||
Catechol and conjugates 4-OHE1/2, 4-OCH3E1/2 (group total) |
– | 1.53±0.31, 28.95±1.56 (30.51) |
1.55±0.17, 28.60±0.61 (30.05) |
1.40±0.10, 27.71±0.74 (29.11) |
1.21±0.08, 28.35±0.73 (29.56) |
1.71±0.12, 68.92±1.95 (70.63) |
2.44±0.35, 71.71±2.33 (74.15) |
0.93±0.24, 25.66±0.97 (26.59) |
1.02±0.23, 24.91±0.67 (25.93) |
0.99±0.10, 19.46±3.45 (20.45) |
0.95±0.01, 21.04±3.59 (21.99) |
3 | 2.49±0.11, 0.62±0.26 (3.11) |
2.21±0.15, 0.71±0.16 (2.92) |
2.44±0.05, 0.17±0.04 (2.61) |
2.43±0.07, 0.18±0.04 (2.61) |
2.19±0.03, 0.66±0.09 (2.85) |
3.40±0.20, 0.41±0.03 (3.81) |
1.56±0.38 0.12±0.04 (1.68) |
1.51±0.37, 0.20±0.03 (1.71) |
1.82±0.44, 0.33±0.09 (2.15) |
1.64±0.40, 0.23±0.05 (1.87) |
|
Quinone conjugates (4-OHE1/2-2-SG, 4-OHE1/2-2-NAcCys, 4-OHE1/2-2-Cys) (group total) |
– | 0.32±0.02, 0.46±0.03, 0.74±0.05 (1.52) |
0.28±0.03, 0.45±0.02, 0.89±0.04 (1.62) |
0.21±0.04, 0.45±0.02, 0.77±0.03 (1.43) |
0.14±0.07, 0.47±0.03, 0.52±0.12 (1.13) |
0.19±0.01, 0.29±0.03, 0.70±0.02 (1.18) |
0.23±0.02, 0.35±0.08, 0.77±0.03 (1.35) |
0.27±0.01, 0.39±0.02, 0.71±0.02 (1.37) |
0.30±0.05, 0.51±0.03, 0.79±0.03 (1.60) |
0.32±0.02, 0.76±0.03, 0.71±0.02 (1.79) |
0.24±0.03, 0.59±0.05, 0.71±0.03 (1.54) |
3 | 0.48±0.1, 0.62±0.05, 1.43±0.09 (2.53) |
0.48±0.02, 0.61±0.01, 1.37±0.07 (2.46) |
0.34±0.02, 0.63±0.02, 1.31±0.04 (2.28) |
0.27±0.07, 0.67±0.06, 0.61±0.13 (1.55) |
0.26±0.10 0.81±0.07, 1.00±0.07 (2.07) |
0.32±0.02, 0.80±0.08, 1.01±0.10 (2.13) |
0.30±0.02, 0.75±0.02, 1.14±0.13 (2.19) |
0.35±0.02, 0.81±0.02, 1.10±0.06 (2.26) |
0.41±0.03, 0.98±0.01, 1.04±0.10 (2.43) |
0.37±0.04, 1.04±0.09, 0.94±0.09 (2.35) |
|
DNA adducts 4-OHE1/2-1-N7Gua, 4-OHE1/2-1-N3Ade (group total) |
– | 0.15±0.01, 0.13±0.01 (0.28) |
0.13±0.02, 0.14±0.03 (0.27) |
0.16±0.01, 0.14±0.03 (0.30) |
0.13±0.01, 0.10±0.03 (0.23) |
0.09±0.01, 0.08±0.02 (0.17) |
0.10±0.01, 0.09±0.01 (0.19) |
0.21±0.01, 0.21±0.04 (0.42) |
0.25±0.07, 0.24±0.07 (0.49) |
0.27±0.09, 0.25±0.08 (0.52) |
0.24±0.07, 0.24±0.07 (0.48) |
3 | 0.54±0.02, 0.58±0.02 (1.12) |
0.50±0.06 0.54±0.03 (1.04) |
0.52±0.09, 0.50±0.04 (1.02) |
0.49±0.05, 0.47±0.06 (0.96) |
0.37±0.10, 0.33±0.07 (0.70) |
0.36±0.07, 0.36±0.06 (0.72) |
0.65±0.07, 0.66±0.07 (1.31) |
0.60±0.05, 0.62±0.02 (1.22) |
0.89±0.19, 0.85±0.15 (1.74) |
0.73±0.12, 0.76±0.06 (1.49) |