FIG. 3.

Chemoresistance of RW-GFP cells. (A, B) RWPE-1 and RW-GFP cells were untreated (−Dox) or treated (+Dox) with doxorubicin (Dox, 0.5 μg/mL) for 48 h, and (A) immunoblot analysis was then performed on whole cell lysate for p53 expression, using anti-β-actin antibodies as a loading control; (B) cells were photographed (scale bar=100 μm), (C, D) RWPE-1 and RW-GFP cells were untreated or treated with arsenite (5, 10, 20, 30, 40 μM) for 48 h and (C) viable cells were counted using the trypan blue dye exclusion method, and (D) cells were photographed (scale bar=100 μm); (C) Data represent mean values±standard error of the mean of five independent experiments; P values were determined by Student's test **P<0.01, *P<0.05.