FIG. 4.

RW-GFP cells exhibit a higher expression level of stem cell markers and a significant increase of long noncoding RNA H19 expression. (A) Fold-enrichment over the parental RWPE-1 cells for CD44, CD166, and TROP2 cell surface marker expression analyzed by flow cytometry; data are expressed as mean fluorescence intensity (MFI) of RW-GFP cells (black bars) as compared with parental RWPE-1 cells (open bars). (B) Expression of stemness genes and (C) long noncoding RNA H19 was analyzed by qRT-PCR using total RNA from parental RWPE-1 cells (open bars) or RW-GFP cells (closed bars). Levels were normalized to those of RPLP0 internal control. Graphs show fold enrichment in RW-GFP cells over the parental RWPE-1 cells for each gene. (A, B, C) Data represent mean values±standard error of the mean of three independent experiments; P values were determined by Student's test ***P<0.001, **P<0.01, and *P<0.05.