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. 2015 Apr 30;9:2485–2499. doi: 10.2147/DDDT.S82030

Figure 6.

Figure 6

Western blot analysis of the effect of BPC-157 on the ERK1/2, JNK, and p38 phosphorylation levels.

Notes: HUVECs were synchronized in medium for 24 hours and then treated with or without 1 μg/mL, 5 μg/mL, or 10 μg/mL BPC-157 for another 1 hour. Immunoblotting was performed three to four times using independently prepared cell lysates, and protein levels for p-ERK1/2, total ERK, p-JNK, total JNK, p-p38, total p38, and tubulin in every group and their corresponding relative densities are shown.

Abbreviations: BPC-157, body protective compound 157; ERK1/2, extracellular signal-regulated kinases 1 and 2; HUVECs, human umbilical vein endothelial cells.