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. 2015 Apr 30;9:2485–2499. doi: 10.2147/DDDT.S82030

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© 2015 Huang et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Effects of MAPK inhibitors on BPC-157-induced cell viability, migration, and tube formation of HUVECs.

Notes: (A) Effect of MAPK inhibitors on BPC-157-induced cell proliferation of HUVECs. Cell viability was determined by MTT assay after treatment with 10 μg/mL of BPC-157 for 72 hours in the presence or absence of inhibitors for ERK, JNK, or p38 MAPK (10 μM, 10 μM, and 10 μM, respectively). (B) Effect of MAPK inhibitors on BPC-157-induced cell migration of HUVECs. HUVECs were pretreated with 10 μg/mL BPC-157 for 24 hours in the presence or absence of inhibitors for ERK, JNK, or p38 MAPK (10 μM, 10 μM, and 10 μM, respectively). Migrated cells were stained with DAPI. Photographs of the migration HUVECs were taken under a microscope. (C) Quantification of cell migration in HUVECs is shown. Positive (+) and negative (-) symbols refer to the presence and absence of the treatment, respectively. (D) Effect of MAPK inhibitors on BPC-157-induced wound healing migration of HUVECs. HUVECs were incubated with 10 μg/mL BPC-157 for 12 hours in the presence or absence of inhibitors for ERK, JNK, or p38 MAPK (10 μM, 10 μM, and 10 μM, respectively), and the migration distances of cells were calculated. (E) Quantification of cell motility in HUVECs is shown. Positive (+) and negative (-) symbols refer to the presence and absence of the treatment, respectively. (F) Effect of MAPK inhibitors on BPC-157-induced tube formation. HUVECs were pretreated with PD98059 (10 μM), SP600125 (10 μM), and SB203580 (10 μM) for 30 minutes and cultured in the presence of 10 μg/mL BPC-157. After 8 hours of incubation, Matrigel tube formation was evaluated. (G) Number of capillary structures. Data are presented as mean ± SD. Differences between the treated and untreated control groups were determined by Student’s unpaired t-test. *P<0.05 significantly different from the control group. Positive (+) and negative (−) symbols refer to the presence and absence of the treatment, respectively.

Abbreviations: BPC-157, body protective compound 157; DAPI, 4′,6-diamidino-2-phenylindole; ERK, extracellular signal-regulated kinases; HUVECs, human umbilical vein endothelial cells; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; MAPK, mitogen-activated protein kinase; OD, optical density; SD, standard deviation.

Effects of MAPK inhibitors on BPC-157-induced cell viability, migration, and tube formation of HUVECs.

Notes: (A) Effect of MAPK inhibitors on BPC-157-induced cell proliferation of HUVECs. Cell viability was determined by MTT assay after treatment with 10 μg/mL of BPC-157 for 72 hours in the presence or absence of inhibitors for ERK, JNK, or p38 MAPK (10 μM, 10 μM, and 10 μM, respectively). (B) Effect of MAPK inhibitors on BPC-157-induced cell migration of HUVECs. HUVECs were pretreated with 10 μg/mL BPC-157 for 24 hours in the presence or absence of inhibitors for ERK, JNK, or p38 MAPK (10 μM, 10 μM, and 10 μM, respectively). Migrated cells were stained with DAPI. Photographs of the migration HUVECs were taken under a microscope. (C) Quantification of cell migration in HUVECs is shown. Positive (+) and negative (-) symbols refer to the presence and absence of the treatment, respectively. (D) Effect of MAPK inhibitors on BPC-157-induced wound healing migration of HUVECs. HUVECs were incubated with 10 μg/mL BPC-157 for 12 hours in the presence or absence of inhibitors for ERK, JNK, or p38 MAPK (10 μM, 10 μM, and 10 μM, respectively), and the migration distances of cells were calculated. (E) Quantification of cell motility in HUVECs is shown. Positive (+) and negative (-) symbols refer to the presence and absence of the treatment, respectively. (F) Effect of MAPK inhibitors on BPC-157-induced tube formation. HUVECs were pretreated with PD98059 (10 μM), SP600125 (10 μM), and SB203580 (10 μM) for 30 minutes and cultured in the presence of 10 μg/mL BPC-157. After 8 hours of incubation, Matrigel tube formation was evaluated. (G) Number of capillary structures. Data are presented as mean ± SD. Differences between the treated and untreated control groups were determined by Student’s unpaired t-test. *P<0.05 significantly different from the control group. Positive (+) and negative (−) symbols refer to the presence and absence of the treatment, respectively.

Abbreviations: BPC-157, body protective compound 157; DAPI, 4′,6-diamidino-2-phenylindole; ERK, extracellular signal-regulated kinases; HUVECs, human umbilical vein endothelial cells; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; MAPK, mitogen-activated protein kinase; OD, optical density; SD, standard deviation.