The absence of a complex multicellular environment yield enhances proliferation and multilineage potential in neural progenitors. (A): Quantified NS production from prol and differentiating (1, 4, or 7 d) subventricular zone (SVZ)-derived NSCs. (B): 3° sphere formation from 2° spheres generated in (A). (C): Progeny analysis of adherent individual cells expanded clonally for 3 d. (D): Proliferative capacity of individual SVZ cells placed in NS-forming conditions for 3 d. *, p < .05; one-way analysis of variance. Abbreviations: 2°, secondary; 3°, tertiary; d, day; GFAP, glial fibrillary acidic protein; NS, neurosphere; prol, proliferating.