Figure 1. THP1 cells cultured in zinc deficient media had increased cell adherence and decreased total zinc.
THP1 cells were cultured in zinc adequate (ZA) or zinc deficient (ZD) media for up to 25d. (A) Representative images showing ZA and ZD THP1 cell morphology at 3d and 25d. (B) Total zinc in ZA and ZD THP1 cells were determined by ICP-OES after two weeks culture in ZA and ZD media, respectively (n=3 per group). Data represent mean ± SEM. *P < 0.01 vs. ZA. Results are representative of three independent experiments.